6.20-6.23: Screening over the first chemical library (800 candidates)
6.24-5.30: Screening over the Second chemical library (2,000 candidates)
7.10: Optimize the Condition for DSF assay (three factors including protein concentration, dye concentration, and buffer type)
7.14-7.20: Use different Concentrations of protein (5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 uM) with 2.5x SYPRO red dye (final concentration) and found out that 50 uM concentration was suitable for detection.
8.1-8.10: Use different concentrations of fluorescence dye (SYPRO red) to optimize the detection of protein stability shift using 100 uM DNA as a positive control, and found out that 12.5x final concentration is the best.
8.16-8.30: Purchase the top 30 chemical candidates and test them with the system described below and search for possible candidates.